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Image Search Results
Journal: Addiction Biology
Article Title: Dysregulation of the histone demethylase KDM6B in alcohol dependence is associated with epigenetic regulation of inflammatory signaling pathways
doi: 10.1111/adb.12816
Figure Lengend Snippet: Alignment statistics for the H3K27me3 ChIP‐seq analysis
Article Snippet: Specificity of the
Techniques:
Journal: Addiction Biology
Article Title: Dysregulation of the histone demethylase KDM6B in alcohol dependence is associated with epigenetic regulation of inflammatory signaling pathways
doi: 10.1111/adb.12816
Figure Lengend Snippet: KDM6B protein levels are increased, and H3K27me3 levels are decreased in the nucleus accumbens of alcohol‐dependent rats. A,B, KDM6B (A) and H3K27me3 (B) levels relative to ACTB and total histone H3, respectively, in control (CON) compared with postdependent (PD) rat nucleus accumbens (* P < .05, n = 5‐7 CON, n = 9‐10 PD). C,D, KDM6B (C) and H3K27me3 (D) levels in an independent group of rats subjected to the same dependence induction paradigm (* P < .05, *** P < .001, n = 7 CON, n = 7‐8 PD). E, Representative western blot image
Article Snippet: Specificity of the
Techniques: Western Blot
Journal: Addiction Biology
Article Title: Dysregulation of the histone demethylase KDM6B in alcohol dependence is associated with epigenetic regulation of inflammatory signaling pathways
doi: 10.1111/adb.12816
Figure Lengend Snippet: Chromatin immunoprecipitation sequencing (ChIP‐seq) analysis of H3K27me3 in control compared with postdependent (PD) nucleus accumbens. A, A ChIP quantitative real‐time polymerase chain reaction (qPCR) experiment demonstrated that the H3K27me3 ChIP antibody showed high specificity for the target PTM when tested against a panel of spike‐in recombinant nucleosomes bearing related methylation states that were distinguished by primers specific to unique DNA barcodes. B, Optimization of ChIP‐seq protocol in rodent brain shows enrichment of the antibody signals over IgG controls. H3K27me3, a repressive epigenetic modification, is highly associated with a gene that is suppressed in the brain (BRDT) and lowly associated with an actively transcribed gene (ACTB). In contrast, H3K4me3, a mark associated with transcriptional activation, is enriched for ACTB over BRDT. C, ChIP‐seq analysis shows that the majority of H3K27me3‐associated genomic regions (71%) are enriched in control rats as compared with PD rats ( n = 4)
Article Snippet: Specificity of the
Techniques: ChIP-sequencing, Real-time Polymerase Chain Reaction, Recombinant, Methylation, Modification, Activation Assay
Journal: Addiction Biology
Article Title: Dysregulation of the histone demethylase KDM6B in alcohol dependence is associated with epigenetic regulation of inflammatory signaling pathways
doi: 10.1111/adb.12816
Figure Lengend Snippet: Pathway analysis of the genes differentially enriched for H3K27me3 in control compared with postdependent NAc (ChIP‐seq data, n = 4)
Article Snippet: Specificity of the
Techniques: